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Endospore stain on Bacillus subtilis.The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1]
Bacteria produce a single endospore internally. The spore is sometimes surrounded by a thin covering known as the exosporium, which overlies the spore coat. The spore coat, which acts like a sieve that excludes large toxic molecules like lysozyme, is resistant to many toxic molecules and may also contain enzymes that are involved in germination.
Carbol fuchsin is the primary stain used in this method. Endospores are stained red, while the counterstain methylene blue stains the vegetative bacteria blue. Endospores are surrounded by a highly resistant spore coat, which is highly resistant to excessive heat, freezing, desiccation, as well as chemical agents. More importantly, for ...
Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used. [6] Various bacterial spore staining techniques using Kenyon e.g.
As a model organism, B. subtilis is commonly used in laboratory studies directed at discovering the fundamental properties and characteristics of Gram-positive spore-forming bacteria. [29] In particular, the basic principles and mechanisms underlying formation of the durable endospore have been deduced from studies of spore formation in B ...
Bacterial spores have proven to be difficult to stain as they are not permeable to aqueous dye reagents. Endospore staining is particularly useful for identifying endospore-forming bacterial pathogens such as Clostridioides difficile. Prior to the development of more efficient methods, this stain was performed using the Wirtz method with heat ...
Bacillus cereus endospore stain. B. cereus is a rod-shaped bacterium with a Gram-positive cell envelope. ... These bacteria are both spore-forming and biofilm-forming
The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. [1] The primary stain is malachite green , and the counterstain is safranin , which dyes any other bacterial bodies red.