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  2. Vector (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Vector_(molecular_biology)

    A multiple cloning site is located within lacZ-α, and an insert successfully ligated into the vector will disrupt the gene sequence, resulting in an inactive β-galactosidase. Cells containing vector with an insert may be identified using blue/white selection by growing cells in media containing an analogue of galactose . Cells expressing β ...

  3. Insert (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Insert_(molecular_biology)

    The most common technique to add inserts to desired sequences is the use of homologous recombination. [5] This technique has a specific requirement where the insert can only be added after it has been introduced to the nucleus of the cell, which can be added to the genome mostly during the G2 and S phases in the cell cycle. [6]

  4. Cell fusion - Wikipedia

    en.wikipedia.org/wiki/Cell_fusion

    A heterokaryon is the melding of two or more cells into one and it may reproduce itself for several generations. [4] If two of the same type of cells fuse but their nuclei do not fuse, then the resulting cell is called a syncytium. [5] Heterotypic cell fusion occurs between cells of different types.

  5. Mosaic (genetics) - Wikipedia

    en.wikipedia.org/wiki/Mosaic_(genetics)

    True mosaicism should not be mistaken for the phenomenon of X-inactivation, where all cells in an organism have the same genotype, but a different copy of the X chromosome is expressed in different cells. The latter is the case in normal (XX) female mammals, although it is not always visible from the phenotype (as it is in calico cats).

  6. Genetic transformation - Wikipedia

    en.wikipedia.org/wiki/Genetic_transformation

    The chemical method, however, usually does not work well for linear DNA, such as fragments of chromosomal DNA, probably because the cell's native exonuclease enzymes rapidly degrade linear DNA. In contrast, cells that are naturally competent are usually transformed more efficiently with linear DNA than with plasmid DNA.

  7. Introduction to genetics - Wikipedia

    en.wikipedia.org/wiki/Introduction_to_genetics

    Since traits come from the genes in a cell, putting a new piece of DNA into a cell can produce a new trait. This is how genetic engineering works. For example, rice can be given genes from a maize and a soil bacteria so the rice produces beta-carotene , which the body converts to vitamin A. [ 19 ] This can help children with Vitamin A deficiency.

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  9. Non-Mendelian inheritance - Wikipedia

    en.wikipedia.org/wiki/Non-Mendelian_inheritance

    Non-Mendelian inheritance is any pattern in which traits do not segregate in accordance with Mendel's laws. These laws describe the inheritance of traits linked to single genes on chromosomes in the nucleus. In Mendelian inheritance, each parent contributes one of two possible alleles for a trait.