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  2. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    Gram staining is almost always the first step in the identification of a bacterial group. While Gram staining is a valuable diagnostic tool in both clinical and research settings, not all bacteria can be definitively classified by this technique. This gives rise to gram-variable and gram-indeterminate groups.

  3. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed ...

  4. Analytical chemistry - Wikipedia

    en.wikipedia.org/wiki/Analytical_chemistry

    Thus, different components of the mixture move at different speed. Different components of a mixture can therefore be identified by their respective R ƒ values, which is the ratio between the migration distance of the substance and the migration distance of the solvent front during chromatography. In combination with the instrumental methods ...

  5. Mixture - Wikipedia

    en.wikipedia.org/wiki/Mixture

    These terms are derived from the idea that a homogeneous mixture has a uniform appearance, or only one phase, because the particles are evenly distributed. However, a heterogeneous mixture has constituent substances that are in different phases and easily distinguishable from one another. In addition, a heterogeneous mixture may have a uniform ...

  6. Gas chromatography - Wikipedia

    en.wikipedia.org/wiki/Gas_chromatography

    Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture. [1] In preparative chromatography, GC can be used to prepare pure compounds from a mixture. [2] [3] Gas chromatography is also sometimes known as vapor-phase chromatography (VPC), or gas–liquid partition chromatography ...

  7. Paper chromatography - Wikipedia

    en.wikipedia.org/wiki/Paper_chromatography

    Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition.

  8. Thin-layer chromatography - Wikipedia

    en.wikipedia.org/wiki/Thin-layer_chromatography

    Different compounds in the sample mixture travel at different rates due to the differences in their partition coefficients. [10] Different solvents, or different solvent mixtures, gives different separation. [5] The retardation factor (R f), or retention factor, quantifies the results. It is the distance traveled by a given substance divided by ...

  9. Separation process - Wikipedia

    en.wikipedia.org/wiki/Separation_process

    A separation process is a method that converts a mixture or a solution of chemical substances into two or more distinct product mixtures, [1] a scientific process of separating two or more substances in order to obtain purity. At least one product mixture from the separation is enriched in one or more of the source mixture's constituents.