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A method was introduced to hybridize a large number of DNA samples against numerous DNA probes on a single membrane. The samples would need to be separated into individual lanes within the membrane, which would then be rotated to allow simultaneous hybridization with multiple DNA probes. [6]
Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence . [ 1 ]
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
In DNA–DNA hybridization, the percent similarity of DNA between two species is estimated by the reduction in hydrogen bonding between nucleotides of imperfectly complemented heteroduplex DNA (i.e., double stranded DNAs that are experimentally produced from single strands of two different species), compared with perfectly matched homoduplex ...
The dual ligation hybridization assay (DLA) [6] extends the specificity of the hybridization-ligation assay to a specific method for the parent compound. Despite hybridization-ligation assay's robustness, sensitivity and added specificity for the 3'-end of the oligonculeotide analyte, the hybridization-ligation assay is not specific for the 5 ...
In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA, usually 15–10000 nucleotides long, which can be radioactively or fluorescently labeled. HPs can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe. [ 1 ]
DNA computing requires that the self-assembly of the oligonucleotide strands happen in such a way that hybridization should occur in a manner compatible with the goals of computation. The field of DNA computing was established in Leonard M. Adelman's seminal paper. [1] His work is significant for a number of reasons:
Other chip-based methods such as comparative genomic hybridization can detect genomic gains or deletions leading to LOH. SNP arrays, however, have an additional advantage of being able to detect copy-neutral LOH (also called uniparental disomy or gene conversion). Copy-neutral LOH is a form of allelic imbalance.