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The best sputum samples contain very little saliva, [2] as saliva contaminates the sample with oral bacteria. This is especially true for samples for laboratory testing in cytology or microbiology. Specimen adequacy is assessed by the laboratory technologists by examining a Gram stain or cytology stain of the sputum.
Sputum is a thick fluid produced in the lungs and in the adjacent airways. Normally, fresh morning sample is preferred for the bacteriological examination of sputum. [2] A sample of sputum is collected in a sterile, wide-mouthed, dry, leak-proof and break-resistant plastic-container and sent to the laboratory for testing. [2]
To collect the sample, the swab is inserted in the nostril and gently moved forward into the nasopharynx, a region of the pharynx that covers the roof of the mouth. [9] The swab is then rotated for a specified period of time to collect secretions, then the swab is removed and placed into a sterile viral transport media , which preserves the ...
The primary equipment used in BAL includes a fiber-optic bronchoscope, sterile collection traps for collecting test specimens, a sterile saline source, a suction device, and suction tubing. [3] Essentially, the saline source is connected to sterile bronchoscope, as is the specimen collection trap, and then suction tubing is connected to the ...
Sampling of sputum from the lungs for sputum culture. It can be performed by special techniques of coughing, or by a protected specimen brush (PSB), [1] which is a brush that can be retracted into a plastic tube to prevent contamination of bacteria in the throat while inserting and removing the instrument.
Some specimen types also require special treatment, such as immediate mixture with an additive, or storage at a certain temperature. [5] After extraction , all specimen containers must be labeled with at least two of the following identifiers (at the time of collection): patient's name, date of birth, hospital number, test request form number ...
A sputum sample will also be obtained to ascertain the causative bacteria. This also plays a role in informing treatment options. [5] [6] In some cases, pulse oximetry may also be required as pneumonia is known to deplete oxygen levels in the blood. [7] Other vitals such as pulse and body temperature are also checked.
For example, different types of samples must be collected in appropriate tubes to maintain the integrity of the sample and stored at appropriate temperatures (usually 4 °C) to preserve the virus and prevent bacterial or fungal growth. Sometimes multiple sites may also be sampled. [citation needed] Types of samples include the following: