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An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Chromobacterium violaceum is a Gram-negative, facultative anaerobic, non-sporing coccobacillus. It is motile with the help of a single flagellum which is located at the pole of the coccobacillus. Usually, there are one or two more lateral flagella as well. [ 1 ]
TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
The large CV–I complexes become trapped within the gram-positive cell due to the multilayered nature of its peptidoglycan. [18] The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds). [19]
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis, [2] Francisella and Nocardia spp. It contains L-cysteine amino acid and ...
Streak plates of several bacterial species on nutrient agar plates. Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): [1] 0.5% peptone – this provides organic nitrogen
In a sample, E. coli, which is citrate-negative, can be distinguished from non-fecal, citrate-positive coliforms that are often found in water, soil, and on plants using Simmons’ agar. Additionally, Simmons’ agar is commonly used as part of the IMViC tests to identify coliforms.
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