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An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection . [ 1 ]
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis, [2] Francisella and Nocardia spp. It contains L-cysteine amino acid and ...
Staphylococcus growth in Baird-Parker (BP) agar.. Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. [1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci. [1]
Streak plates of several bacterial species on nutrient agar plates. Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): [1] 0.5% peptone – this provides organic nitrogen
The composition of Mueller Hinton agar can affect the growth characteristics of bacterial isolates, as well as their response to antibiotics. For example, variations in the pH of the medium can affect the activity of certain antibiotics, and the presence of certain nutrients can promote the growth of specific bacterial species.