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  2. Multiplex polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Multiplex_polymerase_chain...

    Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform.

  3. Multiple displacement amplification - Wikipedia

    en.wikipedia.org/wiki/Multiple_displacement...

    Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase.

  4. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  5. Loop-mediated isothermal amplification - Wikipedia

    en.wikipedia.org/wiki/Loop-mediated_isothermal...

    Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.

  6. Helicase-dependent amplification - Wikipedia

    en.wikipedia.org/wiki/Helicase-dependent...

    The polymerase chain reaction is the most widely used method for in vitro DNA amplification for purposes of molecular biology and biomedical research. [1] This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying ...

  7. COLD-PCR - Wikipedia

    en.wikipedia.org/wiki/COLD-PCR

    Each double-stranded DNA has a 'critical temperature' (Tc) lower than its Tm. The PCR amplification efficiency drops measurably below the Tc. The Tc is dependent on DNA sequence. Two template DNA fragments differing by only one or two nucleotide mismatches will have different amplification efficiencies if the denaturation step of PCR is set to ...

  8. Random amplification of polymorphic DNA - Wikipedia

    en.wikipedia.org/wiki/Random_amplification_of...

    Random amplified polymorphic DNA (RAPD), pronounced "rapid", [1] is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are random. [2] The scientist performing RAPD creates several arbitrary, short primers (10–12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify.

  9. File:PCR diagrams.pdf - Wikipedia

    en.wikipedia.org/wiki/File:PCR_diagrams.pdf

    Original file (1,275 × 1,752 pixels, file size: 938 KB, MIME type: application/pdf, 3 pages) This is a file from the Wikimedia Commons . Information from its description page there is shown below.

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