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These lenses are often color coded for easier use. The least powerful lens is called the scanning objective lens, and is typically a 4× objective. The second lens is referred to as the small objective lens and is typically a 10× lens. The most powerful lens out of the three is referred to as the large objective lens and is typically 40–100×.
A 40x magnification image of cells in a medical smear test taken through an optical microscope using a wet mount technique, placing the specimen on a glass slide and mixing with a salt solution. Optical microscopy is used extensively in microelectronics, nanophysics, biotechnology, pharmaceutic research, mineralogy and microbiology. [28]
Stepwise magnification by 6% per frame into a 39-megapixel image. In the final frame, at about 170x, an image of a bystander is seen reflected in the man's cornea. Magnification is the process of enlarging the apparent size, not physical size, of something. This enlargement is quantified by a size ratio called optical magnification.
Design features of an operating microscope are: magnification typically in the range from 4x-40x, components that are easy to sterilize or disinfect in order to ensure cross-infection control. There is often a prism that allows splitting of the light beam in order that assistants may also visualize the procedure or to allow photography or video ...
For a single lens surrounded by a medium of refractive index n = 1, the locations of the principal points H and H ′ with respect to the respective lens vertices are given by the formulas = ′ = (), where f is the focal length of the lens, d is its thickness, and r 1 and r 2 are the radii of curvature of its surfaces. Positive signs indicate ...
In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to λ / 2NA , where λ is the wavelength of the light. A lens with a larger numerical aperture will be able to visualize finer details than a lens with a smaller numerical ...
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A telecentric lens is a special optical lens (often an objective lens or a camera lens) that has its entrance or exit pupil, or both, at infinity. The size of images produced by a telecentric lens is insensitive to either the distance between an object being imaged and the lens, or the distance between the image plane and the lens, or both, and ...