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The f-number N is given by: = where f is the focal length, and D is the diameter of the entrance pupil (effective aperture).It is customary to write f-numbers preceded by "f /", which forms a mathematical expression of the entrance pupil's diameter in terms of f and N. [1]
Numerical aperture of a thin lens. Numerical aperture is not typically used in photography. Instead, the angular aperture of a lens (or an imaging mirror) is expressed by the f-number, written f /N, where N is the f-number given by the ratio of the focal length f to the diameter of the entrance pupil D: =.
The original application called for placing the chart at a distance 26 times the focal length of the imaging lens used. The bars above and to the left are in sequence, separated by approximately the square root of two (12, 17, 24, etc.), while the bars below and to the left have the same separation but a different starting point (14, 20, 28, etc.)
Here NA is the numerical aperture, is half the included angle of the lens, which depends on the diameter of the lens and its focal length, is the refractive index of the medium between the lens and the specimen, and is the wavelength of light illuminating or emanating from (in the case of fluorescence microscopy) the sample.
A more typical consumer zoom will have a variable maximum relative aperture since it is harder and more expensive to keep the maximum relative aperture proportional to the focal length at long focal lengths; f /3.5 to f /5.6 is an example of a common variable aperture range in a consumer zoom lens.
The angular aperture of a thin lens with focal point at F and an aperture of diameter . The angular aperture of a lens is the angular size of the lens aperture as seen from the focal point: = (/) = where
Similarly, a point source of light placed at the focal point will produce a collimated beam emanating from the opposite side of the lens, and the lens's large ratio of diameter to focal length (large numerical aperture) allows more light to be captured than would be possible with other spherical lenses. This makes ball lenses particularly ...
Numerical aperture for microscope lenses typically ranges from 0.10 to 1.25, corresponding to focal lengths of about 40 mm to 2 mm, respectively. Mechanical tube length [ edit ]