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Engineering of metabolic processes have been achieved through cell-free systems. [ 24 ] [ 10 ] [ 3 ] Bujara et al. , for example, were able to use glycolytic network extracts, consisting of enzymes from E. coli that produced dihydroxyacetone phosphate , to analyze in real-time the metabolite concentrations while altering enzyme levels, with the ...
Cell-free protein synthesis, also known as in vitro protein synthesis or CFPS, is the production of protein using biological machinery in a cell-free system, that is, without the use of living cells. The in vitro protein synthesis environment is not constrained by a cell wall or homeostasis conditions necessary to maintain cell viability. [ 1 ]
Engineering biology is the set of methods for designing, building, and testing engineered biological systems which have been used to manipulate information, construct materials, process chemicals, produce energy, provide food, and help maintain or enhance human health and environment. [1]
Synthetic biology (SynBio) is a multidisciplinary field of science that focuses on living systems and organisms, and it applies engineering principles to develop new biological parts, devices, and systems or to redesign existing systems found in nature.
Biological engineering is a science-based discipline founded upon the biological sciences in the same way that chemical engineering, electrical engineering, and mechanical engineering [7] can be based upon chemistry, electricity and magnetism, and classical mechanics, respectively.
Cell-free protein array technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This method of synthesizing protein microarrays overcomes the many obstacles and challenges faced by traditional methods of protein array production [1] that have prevented widespread adoption of protein microarrays in proteomics.
Micro-mass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. A commonly applied definition of tissue engineering, as stated by Langer [3] and Vacanti, [4] is "an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve [Biological tissue] function or a ...
Molecular engineering is an emerging field of study concerned with the design and testing of molecular properties, behavior and interactions in order to assemble better materials, systems, and processes for specific functions.