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Various labeling techniques such as enzymatic labeling, protein labeling, and genetic labeling are widely utilized. Ethidium bromide , fluorescein and green fluorescent protein are common tags. The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes for detection of a ...
SNAP-tag reaction scheme. SNAP-tag® is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residue polypeptide (19.4 kDa) that can be fused to any protein of interest and further specifically and covalently tagged with a suitable ligand, such as a fluorescent dye.
FluoProbes dyes are typically used to label proteins or nucleic acids (ultrafast -3 minutes- labeling of antibodies employs Lightning technology [3]). Labeled products can be used for multiparameter detections, life time resolved fluorescence (TRF) , polarisation anisotropy fluorescence , FRET , Quenching, FRAP .
A cyanobacterium stained green with cyanine dye. Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: flow cytometry, microscopy (mainly the visible range, but also UV and IR), microplate assays, microarrays, as well ...
The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology . [ 1 ]
The reporter tag is used to confirm labeling of the enzyme with the reactive group and can vary depending on the downstream readout. The most widely used reporters are fluorescent moieties that enable imaging and affinity tags, such as biotin, that allow for pull-down of labeled enzymes and analysis via mass spectrometry.
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. [1]
A new peptide, known as the Collagen Hybridizing Peptide, can also be conjugated with fluorophores and used to stain denatured collagen fibers. Staining of the plant cell walls is performed using stains or dyes that bind cellulose or pectin. The quest for fluorescent probes with a high specificity that also allow live imaging of plant cells is ...
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