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There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]
Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
In computer science and graph theory, the maximum weight matching problem is the problem of finding, in a weighted graph, a matching in which the sum of weights is maximized. A special case of it is the assignment problem , in which the input is restricted to be a bipartite graph , and the matching constrained to be have cardinality that of the ...
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
The matching problem can be generalized by assigning weights to edges in G and asking for a set M that produces a matching of maximum (minimum) total weight: this is the maximum weight matching problem. This problem can be solved by a combinatorial algorithm that uses the unweighted Edmonds's algorithm as a subroutine. [6]
The size of restriction fragments determines the resolution of interaction mapping. Restriction enzymes (REs) that make cuts on 6bp recognition sequences, such as EcoR1 or HindIII, are used for this purpose, as they cut the genome once every 4000bp, giving ~ 1 million fragments in the human genome.
Maximum subarray problems arise in many fields, such as genomic sequence analysis and computer vision.. Genomic sequence analysis employs maximum subarray algorithms to identify important biological segments of protein sequences that have unusual properties, by assigning scores to points within the sequence that are positive when a motif to be recognized is present, and negative when it is not ...
The entire set of fragments must be cloned together with the vector, and separation of clones can occur after. In either case, the fragments are ligated into a vector that has been digested with the same restriction enzyme. The vector containing the inserted fragments of genomic DNA can then be introduced into a host organism. [1]