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Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio.
Mike Anthony Gipson (born June 25, 1966) is an American politician currently serving in the California State Assembly.He is a Democrat representing the 65th Assembly District, which encompasses portions of the South Bay region of Los Angeles County, including Compton, Carson, Willowbrook, and the Watts neighborhood of Los Angeles.
California's 65th State Assembly district is one of 80 California State Assembly districts. It is currently represented by Democrat Mike Gipson and was previously represented by Sharon Quirk-Silva of Fullerton .
In the race to represent the northwestern L.A. County swing district in the state Assembly, Patrick Lee Gipson aims to flip Democrat Pilar Schiavo's seat back to Republicans.
The Assembly convenes, along with the State Senate, at the California State Capitol in Sacramento. Neither house has been expanded since the ratification of the 1879 Constitution , [ 1 ] and each of the 80 members represent at least 465,000 people, more than any other state lower house, [ citation needed ] and second largest of any lower house ...
2024 California's 1st State Assembly district election [28] [29] Primary election Party Candidate Votes % Republican: Heather Hadwick: 41,939 35.6 Republican: Tenessa Audette 34,439 29.2 Republican: Mark Mezzano 30,090 25.5 Republican: Melissa Hunt 11,344 9.6 Total votes 117,812 : 100.0 : General election Republican: Heather Hadwick: 125,387 : ...
The Gibson assembly method is a relatively straightforward DNA assembly method, requiring only a few additional reagents: the 5' T5 exonuclease, Phusion DNA polymerase, and Taq DNA ligase. The DNA fragments to be assembled are synthesised to have overlapping 5' and 3' ends in the order that they are to be assembled in.
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