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The solid phase now bears a dipeptide. This cycle is repeated to form the desired peptide chain. After all reactions are complete, the synthesised peptide is cleaved from the bead. The protecting groups for the amino groups mostly used in the peptide synthesis are 9-fluorenylmethyloxycarbonyl group and t-butyloxycarbonyl . A number of amino ...
Scheme of solid-phase peptides synthesis (SPPS) on a resin as solid support with protected amino acids.The deprotection is usually done using a base such as piperidine.This is followed by a coupling step (a protected amino acid is added) to the growing peptide chain.
Manual split and pool synthesizer. The device is an aluminum tube mounted on a laboratory shaker and evacuated by a water pump. The split and pool synthesis was first applied to prepare peptide libraries on solid support. The synthesis was realized in a home-made manual device shown in the figure.
Due to the preference for a cis-amide bond [10] with the preceding residue of C2-substituted pseudoprolines, their incorporation results in a kink conformation of the peptide backbone, thus preventing peptide aggregation, self-association, or β-structure formation. Strategies for the use of Pseudoproline in Solid-Phase Peptide Synthesis (SPPS).
HBTU (Hexafluorophosphate Benzotriazole Tetramethyl Uronium) is a coupling reagent used in solid phase peptide synthesis. It was introduced in 1978 and shows resistance against racemization. [3] [4] It is used because of its mild activating properties. [5]
A peptide library is a tool for studying proteins. Peptide libraries typically contain a large number of peptides that have a systematic combination of amino acids. Usually, solid phase synthesis, e.g. resin as a flat surface or beads, is used for peptide library generation.
Merrifield Resin is a cross-linked polystyrene resin that carries a chloromethyl functional group. Merrifield resin is named after its inventor, Robert Bruce Merrifield (1984 winner of the Nobel Prize in Chemistry), and used in solid-phase synthesis. The material is typically available as white beads.
Metallopeptides (also called metal-peptides or metal peptide complexes) are peptides that contain one or more metal ions in their structure. This specific type of peptide are, just like metalloproteins, metallofoldamers. And very similar to metalloproteins, metallopeptide's functionality is attibuted through the contained metal ion cofactor.
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