Search results
Results from the WOW.Com Content Network
LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Ampicillin is used to treat infections by many gram-positive and gram-negative bacteria. It was the first "broad spectrum" penicillin with activity against gram-positive bacteria, including Streptococcus pneumoniae , Streptococcus pyogenes , some isolates of Staphylococcus aureus (but not penicillin-resistant or methicillin-resistant strains ...
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria. [1]: 149 [2]
Ampicillin-sulbactam only comes in a parenteral formulation to be either used as intravenous or intramuscular injections, and can be formulated for intravenous infusion. [2] [17] It is formulated in a 2:1 ratio of ampicillin:sulbactam. The commercial preparations available include: [17] 1.5 grams (1 gram ampicillin and 0.5 gram sulbactam)
Negative selection through replica plating to screen for ampicillin sensitive colonies. Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary ...
Plate 1:10 and 1:100 dilutions of the incubated cultures on selective/ screening plates (e.g. Ampicillin and/or X-gal) onto LB plates to which the antibiotics to be used for selection have been added; Incubate overnight at 37°C; Finally, observe isolated colonies on the plates
It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB medium (lysogeny broth). Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids. [1] [2] SOC medium can also be used to regenerate Klebsiella oxytoca strains for the improved transformation efficiency.
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent.