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Colonies of Micrococcus luteus on Tryptic Soy Agar. Cultivation 48 hours, 37°C. Trypticase soy agar or Tryptic soy agar (TSA) is a growth media for the culturing of moderately to non fastidious bacteria. It is a general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
An agar plate containing tryptone supporting growth of a micro-organism. Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. [1] Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2]
Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious ).
'Lab Lemco' Powder is a refined meat extract that is very light in color and has been in production since 1865. [1] This product is used in a wide range of bacteriological growth media.
In alternative manner, live bacteria cultivated on trypticase soy agar plates may be prepared using sterile technique with a single-line streak inoculation. The inoculated plates are incubated at 37 °C for 24–48 hours to establish colonies. Fresh bacterial preparations should be used.
Cystine tryptic agar (CTA), also known as cystine trypticase agar, [1] [2] is a growth medium used for the identification of microorganisms. [ 3 ] It can be used to determine if organisms can ferment various carbohydrates , including maltose , lactose , and sucrose .
Lauryl tryptose broth (LTB) is a selective growth medium for coliforms. [1]Lauryl tryptose broth is used for the most probable number test of coliforms in waters, effluent or sewage.
Tryptic soy-serum-bacitracin-vancomycin (TSBV) is a type of agar plate medium used in microbiological testing to select for Aggregatibacter actinomycetemcomitans (A. a.). [1] It was described by Jørgen Slots in 1982, who also discovered the role of A.a. in periodontitis. [2] Per litre, TSBV contains: [3] 40 g tryptic soy agar; 1 g yeast extract