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Time-resolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. Here, the fluorescence of a sample is monitored as a function of time after excitation by a flash of light. The time resolution can be obtained in a number of ways, depending on the required sensitivity and time resolution:
Long-lived fluorophores, such as lanthanides, combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interference. This method (commonly referred to as time-resolved fluorometry or TRF) involves two fluorophores: a donor and an acceptor.
Fluorescence lifetimes can be determined in the time domain by using a pulsed source. When a population of fluorophores is excited by an ultrashort or delta pulse of light, the time-resolved fluorescence will decay exponentially as described above. However, if the excitation pulse or detection response is wide, the measured fluorescence, d(t ...
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
In chemistry, the method is more often referred to as fluorescence spectroscopy, but the instrumentation is the same. The relaxation processes can be studied using time-resolved fluorescence spectroscopy to find the decay lifetime of the photoluminescence.
Ultrafast laser spectroscopy is a category of spectroscopic techniques using ultrashort pulse lasers for the study of dynamics on extremely short time scales (attoseconds to nanoseconds). Different methods are used to examine the dynamics of charge carriers, atoms, and molecules.
Ultrafast and time-resolved spectroscopic techniques, including transient absorption and time-resolved fluorescence spectroscopy, allows determination of the rates of singlet exciton decay and the formation of triplet states. Transient absorption techniques capture the rapid conversion of singlet excitons into triplet pairs, highlighting the ...
Time-domain diffuse optics [1] or time-resolved functional near-infrared spectroscopy is a branch of functional near-Infrared spectroscopy which deals with light propagation in diffusive media. There are three main approaches to diffuse optics namely continuous wave [ 2 ] (CW), frequency domain [ 3 ] (FD) and time-domain [ 4 ] (TD).
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