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Dexter (also known as Dexter exchange or collisional energy transfer, colloquially known as Dexter Energy Transfer) is another dynamic quenching mechanism. [12] Dexter electron transfer is a short-range phenomenon that falls off exponentially with distance (proportional to e −kR where k is a constant that depends on the inverse of the van der Waals radius of the atom [citation needed]) and ...
This effect can be used to study molecular geometry and motion. An example of its use is in TaqMan or invader assay, SNP genotyping methods. For instance, a hairpin loop with a fluorophore and quencher at the base of the stem is used. An unlabeled SNP specific PCR primer (one of many) with a specific 5' tail binds to the sequence to be probed ...
Quenching can also be accomplished using inert gases, such as nitrogen and noble gases. Nitrogen is commonly used at greater than atmospheric pressure ranging up to 20 bar absolute. Helium is also used because its thermal capacity is greater than nitrogen.
Instead of a mechanical stopping system, the reaction is halted by quenching, where the products are immediately stopped by freezing, chemical denaturation, or exposure to a denaturing light source. Similar to the continuous-flow method, the time between mixing and quenching can be adjusted by varying the length of the reaction tube.
List of medical abbreviations: Overview; List of medical abbreviations: Latin abbreviations; List of abbreviations for medical organisations and personnel; List of abbreviations used in medical prescriptions; List of optometric abbreviations
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay , that consists of a simple prepare and read method, without the requirement of separation or washing steps.
It is used predominantly as a reducing agent in organic synthesis. The compound features a tetrahedral aluminium center attached to two hydride and two alkoxide groups, the latter derived from 2-methoxyethanol. Commercial solutions are colorless/pale yellow and viscous.
TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...