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Different colors of bromothymol blue at marked pH conditions. Bromothymol blue may be used for observing photosynthetic activities, or as a respiratory indicator (turns yellow as CO 2 is added). [7] [8] A common demonstration of BTB's pH indicator properties involves exhaling through a tube into a neutral solution of BTB.
The photosynthetic efficiency (i.e. oxygenic photosynthesis efficiency) is the fraction of light energy converted into chemical energy during photosynthesis in green plants and algae. Photosynthesis can be described by the simplified chemical reaction 6 H 2 O + 6 CO 2 + energy → C 6 H 12 O 6 + 6 O 2
Elodea is a genus of eight species of aquatic plants often called the waterweeds described as a genus in 1803. Classified in the frog's-bit family ( Hydrocharitaceae ), Elodea is native to the Americas [ 1 ] and is also widely used as aquarium vegetation and laboratory demonstrations of cellular activities.
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in ...
The standards used include potassium dichromate (isosbestic points at 339 and 445 nm), bromothymol blue (325 and 498 nm) and congo red (541 nm). The wavelength of the isosbestic point determined does not depend on the concentration of the substance used, and so it becomes a very reliable reference.
In oxygenic photosynthesis, the first electron donor is water, creating oxygen (O 2) as a by-product. In anoxygenic photosynthesis, various electron donors are used. Cytochrome b 6 f and ATP synthase work together to produce ATP (photophosphorylation) in two distinct ways.
The Emerson effect is the increase in the rate of photosynthesis after chloroplasts are exposed to light of wavelength less than 680 nm (deep red spectrum) and more than 680 nm (far red spectrum). When simultaneously exposed to light of both wavelengths, the rate of photosynthesis is higher than the sum of the red light and far red light ...