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The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate
The Chisanbop system. When a finger is touching the table, it contributes its corresponding number to a total. Chisanbop or chisenbop (from Korean chi (ji) finger + sanpŏp (sanbeop) calculation [1] 지산법/指算法), sometimes called Fingermath, [2] is a finger counting method used to perform basic mathematical operations.
W E ♠ K 10 3 ♥ A Q ♥ K J 9 4 ♦ K Q 3 2 ♦ A J ♣ A 4 3 ♣ 8 7 6 5 ♠ A Q J W E ♠ K 10 3 ♥ A Q 3 2 ♥ K J 9 4 ♦ K Q ♦ A J ♣ A 4 3 2 ♣ 8 7 6 5 Both East hands are exactly the same, and both West hands have the same shape, the same HCP count, and the same high cards. The only difference between the West hands is that two low red cards and one low black card have been ...
In 1982 the U.S. Department of Justice Merger Guidelines introduced the SSNIP test as a new method for defining markets and for measuring market power directly. In the EU it was used for the first time in the Nestlé/Perrier case in 1992 and has been officially recognized by the European Commission in its "Commission's Notice for the Definition of the Relevant Market" in 1997.
After a 6-year hiatus, the Ringling Bros. and Barnum & Bailey circus is returning in a revived show that no longer features animal acts. (Photo Illustration: Yahoo News; Photos: Getty Images ...
Processing revealed in 1884 that the ore was also rich in nickel and exploration tests revealed an enormous potential. In 1893 Robert M. Thompson patented the Orford "Tops and Bottoms" process; this was the first commercially viable method of separating (Fe,Ni) 9 S 8 Pentlandite-borne Nickel from the CuFeS 2 Chalcopyrite-borne Copper. [5]