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  2. 1951 USAF resolution test chart - Wikipedia

    en.wikipedia.org/wiki/1951_USAF_resolution_test...

    Glass chart. A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens.

  3. Ocular micrometer - Wikipedia

    en.wikipedia.org/wiki/Ocular_micrometer

    Ocular micrometer Micrometer Eyepiece. An ocular micrometer or eyepiece micrometer is a glass disk, engraved with a ruled scale, that fits in an eyepiece of a microscope, [1] [2] which is used to measure the size of microscopic objects through magnification under a microscope.

  4. Filar micrometer - Wikipedia

    en.wikipedia.org/wiki/Filar_micrometer

    A typical filar micrometer consists of a reticle that has two fine parallel wires or threads that can be moved by the observer using a micrometer screw mechanism. The wires are placed in the focal image plane of the eyepiece so they remain sharply superimposed over the object under observation, while the micrometer motion moves the wires across ...

  5. Optical sectioning - Wikipedia

    en.wikipedia.org/wiki/Optical_sectioning

    With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning.

  6. Fiducial marker - Wikipedia

    en.wikipedia.org/wiki/Fiducial_marker

    The appearance of markers in images may act as a reference for image scaling, or may allow the image and physical object, or multiple independent images, to be correlated. By placing fiducial markers at known locations in a subject, the relative scale in the produced image may be determined by comparison of the locations of the markers in the ...

  7. Microscope image processing - Wikipedia

    en.wikipedia.org/wiki/Microscope_image_processing

    Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.

  8. Aberration-Corrected Transmission Electron Microscopy

    en.wikipedia.org/wiki/Aberration-Corrected...

    The benefit of the scanning transmission electron microscope (STEM) and its potentional for high-resolution imaging had been investigated by Albert Crewe.He investigated the need for a brighter electron source in the microscope, positing that cold field emission guns would be feasible. [9]

  9. Photoactivated localization microscopy - Wikipedia

    en.wikipedia.org/wiki/Photoactivated...

    The two dimensional image of a point source observed under a microscope is an extended spot, corresponding to the Airy disk (a section of the point spread function) of the imaging system. The ability to identify as two individual entities two closely spaced fluorophores is limited by the diffraction of light.

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