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Lac repressor (LacI) is a protein that regulates the expression of lactose metabolism genes in bacteria. Learn about its function, structure, kinetics, and allosteric regulation by lactose and allolactose.
The T7 expression system is a method to clone recombinant DNA using E. coli and T7 RNA polymerase. It is widely used for expressing recombinant proteins and mRNA vaccines, and was developed at Brookhaven National Laboratory.
Learn how cells control the production of specific gene products (protein or RNA) by modulating various stages of gene expression. Find out how gene regulation is essential for development, evolution, and adaptation, and how it can be influenced by environmental stimuli, hormones, or epigenetic factors.
Toggle switch which operates using two mutually inhibitory genes, each promoter is inhibited by the repressor that is transcribed by the opposing promoter. Toggle switch design: Inducer 1 inactivates repressor 1, which means repressor 2 is produced. Repressor 2, in turn, stops transcription of the repressor 1 gene and the reporter gene. [14]
A repressor is a protein that inhibits gene expression by binding to an operator or mRNA. Learn how repressors work, how they are regulated by inducers or co-repressors, and see examples from lac, met and arabinose operons.
The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria.Although glucose is the preferred carbon source for most enteric bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available through the activity of β-galactosidase. [1]
An operon is a cluster of genes under the control of a single promoter and operator, transcribed together into an mRNA. Learn about the history, types and functions of operons in prokaryotes and eukaryotes, and see the lac operon as an example.
A silencer is a DNA sequence that binds repressors, which are proteins that prevent transcription of a gene. Learn about the types, locations, mechanisms and examples of silencers in eukaryotes and prokaryotes.