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The emulsion test is a simple method used educational settings to determine the presence of lipids using wet chemistry. The procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve (lipids are soluble in alcohols). The liquid (alcohol with dissolved fat) is then decanted into water.
Lipid emulsion or fat emulsion refers to an emulsion of fat for human intravenous use, to administer nutrients to critically-ill patients that cannot consume food. It is often referred to by the brand name of the most commonly used version, Intralipid , which is an emulsion containing soybean oil , egg phospholipids and glycerin , and is ...
Download as PDF; Printable version; ... Lipids are a broad group of organic compounds which include fats, ... Emulsion test; Lipid microdomain;
The Liebermann–Burchard or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive. Lieberman–Burchard is a reagent used in a colourimetric test to detect cholesterol, which gives a deep green colour. This colour begins as a purplish, pink colour and ...
HLB scale showing classification of surfactant function. The hydrophilic–lipophilic balance (HLB) of a surfactant is a measure of its degree of hydrophilicity or lipophilicity, determined by calculating percentages of molecular weights for the hydrophilic and lipophilic portions of the surfactant molecule, as described by Griffin in 1949 [1] [2] and 1954. [3]
The oil is emulsified with detergents using a high-shear mixer to stabilize the emulsion so, when they encounter the lipids in the cell membrane or envelope of bacteria or viruses, they force the lipids to merge with themselves. On a mass scale, in effect this disintegrates the membrane and kills the pathogen.
In multilamellar liposomes, many such lipid bilayer sheets are layered concentrically with water layers in between. Figure 1 Multi-lamellar phase of aqueous lipid dispersions, each white lamella represents a lipid bilayer organization in liposome made by vortex-mixing of dried total lipid extract of spinach thylakoid membranes with distilled water.
Salkowski test can also be used to test the presence of indoles (crystalline alkaloids that are a degradation products of proteins, containing tryptophan [10]). In such cases a sample is treated with nitric acid and 2% solution of potassium nitrite , with positive reaction being shown by presence of red colour.