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African swine fever virus (ASFV) is a large, double-stranded DNA virus in the Asfarviridae family. [1] It is the causative agent of African swine fever (ASF). The virus causes a hemorrhagic fever with high mortality rates in domestic pigs ; some isolates can cause death of animals as quickly as a week after infection.
Virus quantification is counting or calculating the number of virus particles (virions) in a sample to determine the virus concentration. It is used in both research and development (R&D) in academic and commercial laboratories as well as in production situations where the quantity of virus at various steps is an important variable that must be monitored.
Here, k ≈ 1.38 × 10 −23 J/K is the Boltzmann constant and kT 0 is the available noise power density (the noise is thermal noise, Johnson noise). As a numerical example: A receiver has a bandwidth of 100 MHz, a noise figure of 1.5 dB and the physical temperature of the system is 290 K.
As described Balayla et al., [12] repeating the same test may violate the this independence assumption and in fact "A more natural and reliable method to enhance the positive predictive value would be, when available, to use a different test with different parameters altogether after an initial positive result is obtained.".
In statistics, the 68–95–99.7 rule, also known as the empirical rule, and sometimes abbreviated 3sr or 3 σ, is a shorthand used to remember the percentage of values that lie within an interval estimate in a normal distribution: approximately 68%, 95%, and 99.7% of the values lie within one, two, and three standard deviations of the mean ...
The instrument detection limit (IDL) is the analyte concentration that is required to produce a signal greater than three times the standard deviation of the noise level. This may be practically measured by analyzing 8 or more standards at the estimated IDL then calculating the standard deviation from the measured concentrations of those standards.
The predecessor to the CAD, termed an evaporative electrical detector, was first described by Kaufman in 2002 at TSI Inc in US patent 6,568,245 [5] and was based on the coupling of liquid chromatographic approaches to TSI's electrical aerosol measurement (EAM) technology. [6]
A typical molecular beacon structure can be divided in 4 parts: 1) loop, an 18–30 base pair region of the molecular beacon that is complementary to the target sequence; 2) stem formed by the attachment to both termini of the loop of two short (5 to 7 nucleotide residues) oligonucleotides that are complementary to each other; 3) 5' fluorophore ...