Search results
Results from the WOW.Com Content Network
In a direct fluorescent antibody test, antibodies have been chemically linked to a fluorescent dye FISH image of bifidobacteria Cy3 FISH analysis di george syndrome. Fluorescent labeling is known for its non-destructive nature and high sensitivity. This has made it one of the most widely used methods for labeling and tracking biomolecules. [1]
In these embryos the concentration of Bicoid protein could be accurately measured either by the intensity of the autofluorescence of the GFP protein itself (not shown) or by the intensity of fluorescence of an anti-GFP antibody tagged with a fluorescent dye, as shown in a surface view of the embryo here.
SNAP-tag reaction scheme. SNAP-tag® is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residue polypeptide (19.4 kDa) that can be fused to any protein of interest and further specifically and covalently tagged with a suitable ligand, such as a fluorescent dye.
A cyanobacterium stained green with cyanine dye. Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: flow cytometry, microscopy (mainly the visible range, but also UV and IR), microplate assays, microarrays, as well ...
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
LifeAct peptides have been used as a universal marker for F-actin visualization in biomedical research. An experiment conducted by Sawant et al. utilized LifeAct GFP to visualize the migration of control border cells in the ovaries of Drosophila flies, in order to determine how cells move in terms of small and large collectives during development and cancer. [6]
In the case of synthetic peptides, the advantage is the amino acid sequence is easily accessible, but the peptides do not always resemble the 3-D structure or post-translational modification found in the native form of the protein. Therefore, antibodies that are produced to work against a synthetic peptide may have problems with the native 3-D ...
Some impermeable fluorescent zinc dyes can detectably label the cytosol and nuclei of apoptizing and necrotizing cells among each of four different tissue types examined. . Namely: the cerebral cortex, the hippocampus, the cerebellum, and it was also demonstrated that colocalized detection of zinc increase and the well accepted cell death indicator propidium iodide also occurred in kidney