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Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition.
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
Spinach (Spinacia oleracea) is a leafy green flowering plant native to central and Western Asia. It is of the order Caryophyllales, family Amaranthaceae, subfamily Chenopodioideae. Its leaves are a common edible vegetable consumed either fresh, or after storage using preservation techniques by canning, freezing, or dehydration.
Paper chromatography of some spinach leaf extract shows the various pigments present in their chloroplasts: yellowish xanthophylls, greenish chlorophylls a and b. Plants and various other groups of photosynthetic eukaryotes collectively known as "algae" have unique organelles known as chloroplasts.
40% of the leaf mass typical for the variety reached 45: 50% of the leaf mass typical for the variety reached 46: 60% of the leaf mass typical for the variety reached 47: 70% of the leaf mass typical for the variety reached 48: 80% of the leaf mass typical for the variety reached 49: Typical leaf mass reached 5: Inflorescence emergence 51
It grows as a trailing undershrub with thick, pale, furry stems, and thick, oval, saddle-shaped leaves 10-60 millimetres long and 5-30 millimetres wide. Flowers occur in clusters of three to five, and comprise four light yellow perianth segments surrounding a centre of many stamens. [ 2 ]
The leaves of the plant are 3–15 cm long, triangular in shape, and bright green. The leaves are thick, and covered with tiny papillae that look like waterdrops on the top and bottom of the leaves. The flowers of the plant are yellow, [ 8 ] and the fruit are small, hard capsules each with 4-10 horned seeds.
Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation technique of gas chromatography with the identification technique of mass spectrometry. GC-MS is the single most important analytical tool for the analysis of volatile and semi-volatile organic compounds in complex mixtures. [7]