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Through near field spectroscopy (NFS), one can probe spectroscopically with sub-wavelength resolution. Raman SNOM and fluorescence SNOM are two of the most popular NFS techniques as they allow for the identification of nanosized features with chemical contrast. Some of the common near-field spectroscopic techniques are below.
The schematic representation of a nano-FTIR system with a broadband infrared source. Nano-FTIR (nanoscale Fourier transform infrared spectroscopy) is a scanning probe technique that utilizes as a combination of two techniques: Fourier transform infrared spectroscopy (FTIR) and scattering-type scanning near-field optical microscopy (s-SNOM).
As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. Also the good optical sectioning capability reduces the background signal and thus creates images with higher contrast, comparable to confocal microscopy. Because light sheet fluorescence microscopy scans samples by ...
Confocal microscopy was then introduced in 1960 which decreased the background and exposure time of the sample by directing light to a pinpoint and illuminating cones of light into the sample. In the 1980s, the introduction of TIRFM further decreased background and exposure time by only illuminating the thin section of the sample being examined ...
Dark-field microscopy produces an image with a dark background Operating principles of dark-field and phase-contrast microscopies Dark-field microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled ...
(May 2020) (Learn how and when to remove this message) Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit , [ 1 ] [ 2 ] which is due to the diffraction of light. [ 3 ]
In its positive form, the background light is instead phase-shifted by +90°. The background light will thus be 180° out of phase relative to the scattered light. The scattered light will then be subtracted from the background light to form an image with a darker foreground and a lighter background, as shown in the first figure. [5] [6] [7]
Snom Technology GmbH / s n ʊ m / is a German company which manufactures Voice over Internet Protocol (VoIP) telephones, based on the IETF standard Session Initiation Protocol (SIP). Snom's products are targeted at the small- to medium-sized business sector, home offices , Internet service providers , carriers , and original equipment ...