Search results
Results from the WOW.Com Content Network
Lysis (/ ˈ l aɪ s ɪ s / LY-sis; from Greek λῠ́σῐς lýsis 'loosening') is the breaking down of the membrane of a cell, often by viral, enzymic, or osmotic (that is, "lytic" / ˈ l ɪ t ɪ k / LIT-ik) mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a lysate.
Sonoporation, or cellular sonication, is the use of sound in the ultrasonic range for increasing the permeability of the cell plasma membrane. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or ...
Sonication can be used for the production of nanoparticles, such as nanoemulsions, [5] nanocrystals, liposomes and wax emulsions, as well as for wastewater purification, degassing, extraction of seaweed polysaccharides [1] and plant oil, extraction of anthocyanins and antioxidants, [6] production of biofuels, crude oil desulphurization, cell disruption, polymer and epoxy processing, adhesive ...
It has since been used in other applications such as cell disruption nanoemulsions, and solid particle size reduction, among others. By using microchannels with fixed geometry, and an intensifier pump, high shear rates are generated that rupture the cells. This method of cell lysis can yield breakage of over 90% of E. coli cells. [9]
The protein manufacturing cost remains high and there is a growing demand to develop cost efficient and rapid protein purification methods. Understanding the different protein purification methods and optimizing the downstream processing is critical to minimize production costs while maintaining the quality of acceptable standards of homogeneity. [2]
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
The cell type of interest is engineered to express a ribosomal subunit fused to an epitope tag such as green fluorescent protein. [10] After cell lysis, antibodies targeting the epitope are used to isolate mRNAs that are bound to the ribosomes containing the fusion proteins. This RNA is then converted to cDNA and sequenced. This technique ...
Once the cell is lysed, the bacteriophage is able to release progeny virions into the environment which in turn infect more bacterial cells. [5] In addition to degrading bacterial cell walls from within, endolysins are effective when applied externally and can lyse Gram-positive bacteria that lack an outer cell membrane . [ 6 ]