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The procedure entails using a syringe to collect synovial fluid from or inject medication into the joint capsule. Laboratory analysis of synovial fluid can further help characterize the diseased joint and distinguish between gout, arthritis, and synovial infections such as septic arthritis. [citation needed]
Arthrocentesis, or removing synovial fluid from the affected joint, is performed to test the synovial fluid for the calcium pyrophosphate crystals that are present in CPPD. When stained with H&E stain , calcium pyrophosphate crystals appears deeply blue (" basophilic ").
Synovial fluid, also called synovia, is a viscous, non-Newtonian fluid found in the cavities of synovial joints. With its egg white –like consistency, [ 1 ] the principal role of synovial fluid is to reduce friction between the articular cartilage of synovial joints during movement. [ 2 ]
Knee effusion, informally known as water on the knee, occurs when excess synovial fluid accumulates in or around the knee joint. It has many common causes, including arthritis , injury to the ligaments or meniscus , or fluid collecting in the bursa , a condition known as prepatellar bursitis .
The surface of the funnel assembly that is in contact with the slide is lined with filter paper to absorb excess fluid. A few drops of fluid are placed in the funnel. The assembly is placed in the cytocentrifuge, which operates at a low force (600–800 x g) to preserve cellular structure. [ 4 ]
Synovial fluid examination [3] [4]; Type WBC (per mm 3) % neutrophils Viscosity Appearance Normal <200: 0: High: Transparent Osteoarthritis <5000 <25: High: Clear yellow Trauma <10,000
Synovial fluid examination [1] [2]; Type WBC (per mm 3) % neutrophils Viscosity Appearance Normal <200: 0: High: Transparent Osteoarthritis <5000 <25: High: Clear yellow Trauma <10,000
The definitive diagnosis is isolation of the causative organism from the synovial fluid (joint fluid), but signs of inflammation in the joint fluid and imaging may also aid in the diagnosis. The treatment is a combination of systemic antibiotics , debridement of infectious and necrotic tissue and local antibiotics applied to the joint space.