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Log reduction is a measure of how thoroughly a decontamination process reduces the concentration of a contaminant. It is defined as the common logarithm of the ratio of the levels of contamination before and after the process, so an increment of 1 corresponds to a reduction in concentration by a factor of 10.
In microbiology, in the context of a sterilization procedure, the D-value or decimal reduction time (or decimal reduction dose) is the time (or dose of an antimicrobial drug) required, at a given condition (e.g. temperature) or set of conditions, to achieve a one-log reduction, that is, to kill 90% of relevant microorganisms. [1]
Z-value is a term used in microbial thermal death time calculations. It is the number of degrees the temperature has to be increased to achieve a tenfold (i.e. 1 log 10 ) reduction in the D-value . The D-value of an organism is the time required in a given medium, at a given temperature, for a ten-fold reduction in the number of organisms.
What is often called a "log reduction" (technically a reduction by one order of magnitude) represents a 90% reduction in microbial population. Thus a process that achieves a "6-log reduction" (10 −6 ) will theoretically reduce an initial population of one million organisms to very close to zero.
Similarly, at larger depths, silt and sand particles may crush and cause a reduction in pore spaces, reducing the biological activity. Bacterial activity is also impacted by challenges such as predation, competition, pH, temperature, and nutrient availability. [55] These factors can contribute to the population decline of bacteria.
Bacterial activity in sewers – anaerobic sulfate reduction at work in the organic-rich sludges accumulated under water in the conduits produces hydrogen sulfide gas (H 2 S). After its released in the air of the galleries, H 2 S is further oxidized into sulfuric acid by atmospheric oxygen .
Mixing the two compounds forms a barium sulfate precipitate, which causes turbidity in the solution. A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4 ).
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent.