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There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University , and it can be prepared in pH 2.2 to 8 by mixing two stock solutions.
This mixture consists of 0.0286 M citric acid, 0.0286 M monopotassium phosphate, 0.0286 M boric acid, 0.0286 M veronal and 0.0286 M hydrochloric acid titrated with 0.2 M sodium hydroxide. The buffer was invented in 1931 by the English chemist Hubert Thomas Stanley "Kevin" Britton (1892–1960) and the New Zealand chemist Robert Anthony Robinson ...
Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction; Reducing surface tension of aqueous solutions during immunostaining (usually at a concentration of 0.1-0.5% in TBS or PBS buffer) Dispersion of carbon materials for soft composite materials; Restricting colony expansion in Aspergillus nidulans in ...
The majority of biological samples that are used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics. They are also used in chemical analysis [5] and calibration of pH ...
MES (2-(N-morpholino)ethanesulfonic acid) is a chemical compound that contains a morpholine ring. It has a molecular weight of 195.2 g/mol and the chemical formula is C 6 H 13 NO 4 S. Synonyms include: 2-morpholinoethanesulfonic acid; 2-(4-morpholino)ethanesulfonic acid; 2-(N-morpholino)ethanesulfonic acid; 2-(4-morpholino)ethanesulfonic acid; MES; MES hydrate; and morpholine-4-ethanesulfonic ...
TE buffer is also known as T 10 E 1 buffer, which can be read as "T ten E one buffer". To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8.
Hanks' salts is a collective group of salts rich in bicarbonate ions, formulated in 1940 by the microbiologist John H. Hanks. [1] Typically, they are used as a buffer system in cell culture media and aid in maintaining the optimum physiological pH (roughly 7.0–7.4) for cellular growth.