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Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.
Cells are adhered to the media that was not removed in a culture vessel, and a series of wash and incubation steps are then necessary to detach the cells. For the wash steps, a balanced salt solution is poured to the side opposite the cell culture, and the culture vessel is then shaken before draining the balanced salt solution.
Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. [1] It is based on six salts and glucose described in Earle's salts in 1934: calcium chloride , potassium chloride , magnesium sulfate , sodium chloride , sodium ...
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
HAT selection depicted by a plasmacytoma thymidine kinase mutant fused with a mortal splenic B-cell.. HAT Medium (hypoxanthine-aminopterin-thymidine medium) is a selection medium for mammalian cell culture, which relies on the combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor by inhibiting dihydrofolate reductase, with hypoxanthine (a purine derivative) and ...
Pages in category "Cell culture media" The following 36 pages are in this category, out of 36 total. This list may not reflect recent changes. A. Antibiotic-Antimycotic;
Certain compounds excreted by plant tissue cultures and found in culture media have been shown necessary to coordinate cell division and morphological changes. [9] These compounds have been identified by Chung et al. [ 10 ] as various polysaccharides , amino acids , growth regulators , vitamins , low molecular weight compounds and polypeptides.