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Cresyl violet stained partial brain section of a Macaque. It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing ...
The Nissl staining technique (named for Franz Nissl the neuroscientist and histologist who originated the technique) is commonly used for determining the cytoarchitectonics of neuroanatomical structures, using common agents such as thionine, cresyl violet, or neutral red.
Photomicrograph of Nissl bodies (two are indicated by arrows) in the cytoplasm of motor neurons in the anterior horn of the spinal cord; cresyl violet stain (purple) along with a luxol fast blue stain for myelin. Scale bar = 30 microns (0.03mm). Drawing of a motor neuron from the ventral horn of the medulla spinals of a rabbit.
Crystal violet stains both Gram positive and Gram negative organisms. Treatment with alcohol removes the crystal violet colour from gram negative organisms only. Safranin as counterstain is used to colour the gram negative organisms that got decolorised by alcohol. While ex vivo, many cells continue to live and metabolize until they are "fixed".
Amyloid plaques are visible with the light microscope using a variety of staining techniques, including silver stains, Congo red, Thioflavin, cresyl violet, PAS-reaction, and luminescent conjugated oligothiophenes (LCOs).
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This is done by using various basic dyes (e.g. aniline, thionine, or cresyl violet) to stain the negatively charged RNA blue, and is used to highlight important structural features of neurons. The Nissl substance ( rough endoplasmic reticulum ) appears dark blue due to the staining of ribosomal RNA, giving the cytoplasm a mottled appearance.
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining.. Basophilic is a technical term used by pathologists. ...