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In microbiology, the minimum inhibitory concentration (MIC) is the lowest concentration of a chemical, usually a drug, which prevents visible in vitro growth of bacteria or fungi. [1] [2] MIC testing is performed in both diagnostic [1] [2] and drug discovery laboratories. [3] [4]
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria. [1]: 149 [2]
If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped bacterial expansion. [1]
Etest is a quantitative technique for determining the antibiotic sensitivity and minimum inhibitory concentration (in μg/mL) of some bacteria including Gram-negative and Gram-positive aerobic bacteria such as Enterobacteriaceae, [2] Pseudomonas, [2] [3] [4] Burkholderia, [2] [5] Staphylococcus, [6] and Enterococcus [7] species and fastidious bacteria, such as anaerobes, N. gonorrhoeae, S ...
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent.
English: Tube Dilution Assay is performed by constantly increasing the percent concentration of antimicrobial agent to microbial rich broth in a series of tubes. It is used to measure the Minimum Inhibitory Concentration [MIC] of an antimicrobial agent, which is the lowest concentration of antimicrobial agent that will inhibit the growth of microbes.
An alternative assessment is to measure the Minimum inhibitory concentrations (MICs) of disinfectants against selected (and representative) microbial species, such as through the use of microbroth dilution testing. [14] However, those methods are obtained at standard inoculum levels without considering the inoculum effect.
The minimum inhibitory concentration (MIC) is then reported as the lowest concentration that produces a clear well and thus inhibits growth. [8] Standardized MIC methods using MHB are not applicable to antimicrobial agents such as defensins, because defensins must be incubated in a low salt buffer, not rich broth, in order to measure their ...