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Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D).
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
A diauxic growth curve refers to the growth curve generated by an organism which has two growth peaks. The theory behind the diauxic growth curve stems from Jacques Monod's Ph.D. research in 1940. A simple example involves the bacterium Escherichia coli ( E. coli ), the best understood bacterium.
μ is the growth rate of a considered microorganism, μ max is the maximum growth rate of this microorganism, [S] is the concentration of the limiting substrate S for growth, K s is the "half-velocity constant"—the value of [S] when μ/μ max = 0.5. μ max and K s are empirical (experimental) coefficients to the Monod equation. They will ...
The name VCC has also been used to describe the application of quantitative growth kinetics to enumerate bacteria in cell culture infection models. [3] Antimicrobial susceptibility testing (AST) can be done on 96-well plates by diluting the antimicrobial agent at varying concentrations in broth inoculated with bacteria and measuring the minimum ...
Subculturing can also be used for growth curve calculations (ex. generation time) [2] and obtaining log-phase microorganisms for experiments (ex. Bacterial transformation). [3] Typically, subculture is from a culture of a certain volume into fresh growth medium of equal volume, this allows long-term maintenance of the cell line.
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature, for cultures from humans or animals, or lower for environmental cultures). After the desired ...
Biological exponential growth is the unrestricted growth of a population of organisms, occurring when resources in its habitat are unlimited. [1] Most commonly apparent in species that reproduce quickly and asexually , like bacteria , exponential growth is intuitive from the fact that each organism can divide and produce two copies of itself.