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The high-density solution may be at a uniform concentration throughout the test tube ("cushion") or a varying concentration ("gradient"). Molecular properties can be modeled through sedimentation velocity analysis or sedimentation equilibrium analysis. During the run, the particle or molecules will migrate through the test tube at different ...
The load in a laboratory centrifuge must be carefully balanced. This is achieved by using a combination of samples and balance tubes which all have the same weight or by using various balancing patterns without balance tubes. [2] It is an interesting mathematical problem to solve the balance pattern given n slots and k tubes with the same weight.
The suspension flows through the filter and the drum with the perforated wall from the inside to the outside. In this way, the solid material is restrained and can be removed. The kind of removing depends on the type of centrifuge, for example manually or periodically. Common types are: Centrifugal oil filters
When blood is collected into a serum-separating tube (SST) and centrifuged, the serum becomes isolated from the red blood cells by a gel acting as a physical barrier to prevent inadvertent remixing of the components. Blood fractionation is the process of fractionating whole blood, or separating it into its component parts.
It uses only gravity to move the mobile phase through the stationary phase which is held in long vertical tubes connected in series. The modern era of CCC began with the development of the planetary centrifuge by Ito which was first introduced in 1966 as a closed helical tube which was rotated on a "planetary" axis as is turned on a "sun" axis.
Samples are placed in tubes within or attached to the rotor. Rotational speed may reach up to 100,000 rpm for floor model, 150,000 rpm for bench-top model (Beckman Optima Max-XP or Sorvall MTX150 or himac CS150NX), creating centrifugal speed forces of 800,000g to 1,000,000g.
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