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The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis.After the sample has been treated to release the hemoglobin from the red cells, it is introduced into a porous gel (usually made of agarose or cellulose acetate) and subjected to an electrical field, most commonly in an alkaline medium.
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A blood smear is made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood over the slide's length. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated.
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Hemoglobin A3 is a predominantly historic term for a fraction of normal hemoglobin molecules that is seen when hemoglobin is separated out using starch block electrophoresis. These Hemoglobin molecules are non-enzymatic modifications of the hemoglobin molecules and they encompass hemoglobin A1c , Hb 1a (both the subtypes Hb 1a1 and Hb 1a2 ) and ...
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A complete blood count (CBC), also known as a full blood count (FBC), is a set of medical laboratory tests that provide information about the cells in a person's blood.The CBC indicates the counts of white blood cells, red blood cells and platelets, the concentration of hemoglobin, and the hematocrit (the volume percentage of red blood cells).