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where ν is the frequency of the wave, λ is the wavelength, ω = 2πν is the angular frequency of the wave, and v p is the phase velocity of the wave. The dependence of the wavenumber on the frequency (or more commonly the frequency on the wavenumber) is known as a dispersion relation.
Spectroscopy can detect a much wider region of the EM spectrum than the visible wavelength range of 400 nm to 700 nm in a vacuum. A common laboratory spectroscope can detect wavelengths from 2 nm to 2500 nm. [1] Detailed information about the physical properties of objects, gases, or even stars can be obtained from this type of device.
The nanometre is often used to express dimensions on an atomic scale: the diameter of a helium atom, for example, is about 0.06 nm, and that of a ribosome is about 20 nm. The nanometre is also commonly used to specify the wavelength of electromagnetic radiation near the visible part of the spectrum: visible light ranges from around 400 to 700 ...
300 nm – greatest particle size that can fit through a HEPA (high efficiency particulate air) filter (N100 removes up to 99.97% at 300 nm, N95 removes up to 95% at 300 nm) [84] 300–400 nm – near ultraviolet wavelength; 400–420 nm – wavelength of violet light (see Color and Visible spectrum) 420–440 nm – wavelength of indigo light
The wavelength of a sine wave, λ, can be measured between any two points with the same phase, such as between crests (on top), or troughs (on bottom), or corresponding zero crossings as shown. In physics and mathematics, wavelength or spatial period of a wave or periodic function is the distance over which the wave's shape repeats.
The relative spectral flux density is also useful if we wish to compare a source's flux density at one wavelength with the same source's flux density at another wavelength; for example, if we wish to demonstrate how the Sun's spectrum peaks in the visible part of the EM spectrum, a graph of the Sun's relative spectral flux density will suffice.
The spectral resolution of a spectrograph, or, more generally, of a frequency spectrum, is a measure of its ability to resolve features in the electromagnetic spectrum.It is usually denoted by , and is closely related to the resolving power of the spectrograph, defined as =, where is the smallest difference in wavelengths that can be distinguished at a wavelength of .
At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base composition.