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Ascorbic acid is easily oxidized and so is used as a reductant in photographic developer solutions (among others) and as a preservative. [citation needed] In fluorescence microscopy and related fluorescence-based techniques, ascorbic acid can be used as an antioxidant to increase fluorescent signal and chemically retard dye photobleaching. [14]
Ascorbic acid (vitamin C) is known to interfere with the oxidation reaction of the blood and glucose pad on common urine test strips. Some urine test strips are protected against the interference with iodate, which eliminates ascorbic acid by oxidation. [25] Some test strips include a test for urinary ascorbate.
Ascorbic acid is a weak sugar acid structurally related to glucose. In biological systems, ascorbic acid can be found only at low pH, but in solutions above pH 5 is predominantly found in the ionized form, ascorbate. [8] Numerous analytical methods have been developed for ascorbic acid detection.
Because the test pad reaction is dependent on pH, false positive results can occur if the urine is highly alkaline. [ 60 ] [ 62 ] Conventional test strips are not sensitive enough to reliably detect microalbuminuria , a condition in which urine albumin levels are slightly elevated, [ 64 ] although dipsticks specialized for this measurement exist.
The following example describes the determination of phosphorus. A sample containing the phosphate is mixed with an acid solution of Mo VI, for example ammonium molybdate, to produce PMo 12 O 3− 40, which has an α-Keggin structure. This anion is then reduced by, for example, ascorbic acid or SnCl 2, to form the blue coloured β-keggin ion ...
[1] [2] If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid. This reaction is a redox reaction: vitamin C (ascorbic acid) is oxidized to dehydroascorbic acid, and DCPIP is reduced to the colorless compound DCPIPH 2
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