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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome . In E. coli , the lacZ gene is the structural gene for β-galactosidase; which is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β ...
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to remove IPTG from the cells and therefore it is difficult to stop expression.
Clues to the existence of a post-mortem transcriptome existed at least since the beginning of the 21st century, [4] but the word thanatotranscriptome (from (thanatos-, Greek for "death") seems to have been first used in the scientific literature by Javan et al. in 2015, [2] following the introduction of the concept of the human thanatomicrobiome in 2014 at the 66th Annual Meeting of the ...
A Florida woman who allegedly snatched a three-year-old boy from his fenced-in yard and ran off down the street last week told the cops she shouldn’t be arrested because she “gave it back ...
2. Excessive Stress. Stress is a natural, normal part of the human experience, and your body knows how to handle it. When you’re under stress, your body releases stress hormones that activate ...
The death-inducing signaling complex (DISC) is a multi-protein complex formed by members of the death receptor family of apoptosis-inducing cellular receptors. [1] A typical example is FasR, which forms the DISC upon trimerization as a result of its ligand binding. The DISC is composed of the death receptor, FADD, and caspase 8. It transduces a ...