Search results
Results from the WOW.Com Content Network
Buoyant density of the majority of DNA is 1.7g/cm 3 [3] which is equal to the density of 6M CsCl solution. [citation needed] Buoyant density of DNA changes with its GC content. The term "satellite DNA" refers to small bands of repetitive DNA sequences with distinct base composition floating above (A+T rich) or below (G+C rich) the main ...
Further application of centrifugation showed that under different conditions the large homogeneous particles could be broken down into discrete subunits. [24] The development of centrifugation was a great advance in experimental protein science. Linderstorm-Lang, in 1937, discovered that density gradient tubes could be used for density ...
Dimensionless numbers (or characteristic numbers) have an important role in analyzing the behavior of fluids and their flow as well as in other transport phenomena. [1] They include the Reynolds and the Mach numbers, which describe as ratios the relative magnitude of fluid and physical system characteristics, such as density, viscosity, speed of sound, and flow speed.
Differential centrifugation, on the other hand, does not utilize a density gradient, and the centrifugation is taken in increasing speeds. The different centrifugation speeds often create separation into not more than two fractions, so the supernatant can be separated further in additional centrifugation steps.
When DNA is extracted from these cells and made to undergo buoyant density centrifugation on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15 N medium had a higher density than cells grown in normal 14 N medium.
In the reference frame spinning with the centrifuge, the centrifugal force induces a hydrostatic pressure gradient in fluid-filled tubes oriented perpendicular to the axis of rotation, giving rise to large buoyant forces which push low-density particles inward. Elements or particles denser than the fluid move outward under the influence of the ...
The FDA rule mandating women be notified of their breast density is the result of intense lobbying by women who found champions in state and federal legislators to get laws introduced and passed.
In the life sciences, a special technique called density gradient separation is used for isolating and purifying cells, viruses and subcellular particles. [5] Variations of this include Isopycnic centrifugation, Differential centrifugation, and Sucrose gradient centrifugation.