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The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The most common application is the separation of solid from highly concentrated suspensions, which is used in ...
Differential centrifugation. In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate.
A centrifuge is a device that uses centrifugal force to subject a specimen to a specified constant force - for example, to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or liquids from solids.
The sedimentation coefficient is typically dependent on the concentration of the solute (i.e. a macromolecular solute such as a protein). Despite 80+ years of study, there is not yet a consensus on the way to perfectly model this relationship while also taking into account all possible non-ideal terms to account for the diverse possible sizes, shapes, and densities of molecular solutes. [2]
Centrifugal force is a fictitious force in Newtonian mechanics ... This is due to the large mass and velocity of the Sun (relative to the Earth).
[b] Centrifugal acceleration is given as rω 2; where r is the radial distance from the rotation axis and ω is the angular velocity in radians per second. Bigger particles tend to sediment faster and so have higher Svedberg values. Svedberg units are not directly additive since they represent a rate of sedimentation, not weight. [1]
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This is the ratio of a particle's sedimentation velocity to the applied acceleration causing the sedimentation. Analytical ultracentrifugation has recently seen a rise in use because of increased ease of analysis with modern computers and the development of software, including a National Institutes of Health supported software package, SedFit.