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The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change. Sometimes, it is possible to detect growth on the Simmons' citrate agar without the accompanying color change to blue. This should be scored as a negative for the citrate use test. [12]
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Use of citrate involves the enzyme citrate lyase, which breaks down citrate to oxaloacetate and acetate. Oxaloacetate is further broken down to pyruvate and carbon dioxide (CO 2 ). Production of sodium bicarbonate (NaHCO 3 ) as well as ammonia (NH 3 ) from the use of sodium citrate and ammonium salts results in alkaline pH.
HCPCS was established in 1978 to provide a standardized coding system for describing the specific items and services provided in the delivery of health care. Such coding is necessary for Medicare , Medicaid , and other health insurance programs to ensure that insurance claims are processed in an orderly and consistent manner.
Healthcare Common Procedure Coding System (including Current Procedural Terminology) (for outpatient use; used in United States) ICD-10 Procedure Coding System (ICD-10-PCS) (for inpatient use; used in United States) ICD-9-CM Volume 3 (subset of ICD-9-CM) (formerly used in United States prior to the introduction of the ICD-10-PCS)
Thiosulfate–citrate–bile salts–sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. [1] TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species.
For example, obligately anaerobic Clostridium species will be seen growing only in the bottom of the test tube. Thioglycolate broth is also used to recruit macrophages to the peritoneal cavity of mice when injected intraperitoneally. [3] It recruits numerous macrophages, but does not activate them. [3]
DCA agar (deoxycholate citrate agar) is a solid bacteriological growth medium used for isolation of enteric pathogens. [1] Uses