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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
The plant tissues are removed from an intact plant in a sterile condition. Clean stock materials that are free of viruses and fungi are important in the production of the healthiest plants. Once the plant material is chosen for culture, the collection of explant(s) begins and is dependent on the type of tissue to be used, including stem tips ...
Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium. [10] The technique of plant tissue culture, i.e., culturing plant cells or tissues in artificial medium supplemented with required nutrients, has many applications in efficient clonal propagation (true to the ...
The tissue must be placed and harvested in an aseptic environment such as sterile laminar flow tissue culture hood. The samples are often minced, and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue onto the surface of the
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The lifespan of most cells is genetically determined, but some cell-culturing cells have been 'transformed' into immortal cells which will reproduce indefinitely if the optimal conditions are provided.
In the case of plant tissue cells, somaclonal variation may arise over long periods in culture. Similarly in mammalian cell lines, chromosomal aberrations have a tendency to increase over time. For microorganisms there is a tendency to adapt to culture conditions, which is rarely precisely like the microorganism's natural environment, which can ...
The cells themselves can either be derived from homogenized tissue or from heterogenous cell solutions. Suspension cell culture is commonly used to culture nonadhesive cell lines like hematopoietic cells, plant cells , and insect cells . [ 1 ]
In botany, the connective is the portion of sterile tissue of the anther between and interconnecting the two thecae, [1] [2] which forms a body with them and holds them together. It is usually underdeveloped and it is subject to great diversity of form, [3] causing the thecae to protrude prominently.