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Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette.The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [1] as it reduces the risk of splashing, foam or bubble formation.
Release the pipette knob slowly to the ready position. Do not remove the tip from the solution. Repeat this process (step 3 and 4) to rinse the tip until the interior wall of the tip is clear. Remove the tip from the solution. Press the pipette knob to the second stop, and completely empty the tip. Release the pipette knob to the ready position.
A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision , from single piece glass pipettes to more complex adjustable or ...
A Serological pipette is designed for use as a blow-out pipette. A Serological pipette also has graduation marks, which start nearer the end of the tip. The pipette can be blown out by gravitational force or air pressure. Rubber bulbs attached to the end opposite the tip are commonly used to "blow out" any remaining solution.
The Kenneth Rainin Foundation was founded in 2007 by Kenneth Rainin, founder of Rainin Instrument Company, which sold a mainstream brand of laboratory pipettes. [ 3 ] [ 4 ] Rainin donated a substantial part of his fortune to a variety of causes during his lifetime.
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
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