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In light microscopy, artifacts may be produced by air bubbles trapped under the slide's cover slip. [1] In electron microscopy, distortions may be produced in the drying out of the specimen. Staining can cause the appearance of solid chemical deposits that may be seen as structures inside the cell.
Critical illumination acts to form an image of the light source on the specimen to illuminate it. [2] This image is formed by the condenser or collector lens . This illumination is bright but not always even, as any structure in the light source (for example the filament of a light bulb ) will be visible in the resulting image.
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.
These are observable under an optical microscope, as they generate near-infrared photons detectable by a CCD camera. Latchups can be observed this way. [9] If visible, the location of failure may present clues to the nature of the overstress.
Most light sheet fluorescence microscopes are used to produce 3D images of the sample by moving the sample through the image plane. If the sample is larger than the field of view of the image sensor, the sample also has to be shifted laterally. An alternative approach is to move the image plane through the sample to create the image stack. [32]
Photic retinopathy is damage to the eye's retina, particularly the macula, from prolonged exposure to solar radiation or other bright light, e.g., lasers or arc welders.The term includes solar, laser, and welder's retinopathy and is synonymous with retinal phototoxicity. [1]
The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...
Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy. Fluorescence itself, is a form of luminescence that results from matter emitting light of a certain wavelength after absorbing electromagnetic radiation. Molecules that re-emit light upon absorption of light are called fluorophores. [1] [2]