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Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
ECACC, which was established in 1984, [1] consists of a team with specialist knowledge which supply authenticated cell lines, induced Pluripotent stem cells (iPSCs) and nucleic acids to provide stock for the research community. ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 ...
Prodiamine is a preemergent herbicide of the dinitroaniline class. Prodiamine is used with crops such as soybeans, alfalfa, cotton, and ornamental crops. Prodiamine inhibits the formation of microtubules. [2] Prodiamine was developed by Sandoz AG and marketed beginning in 1987. [3] Prodiamine can be obtained starting from 2,4 ...
During the same period in the mid 1980s, a monoclonal antibody was reported by the group of Walter Birchmeier to disrupt cell-cell contacts and alter the front-rear polarity of cells in culture. [ 14 ] [ 15 ] The target of this antibody was later identified as a component of cell-cell junctions, E-cadherin . [ 16 ]
This configuration allows for efficient proton conduction and effective gas diffusion, making it suitable for various applications, including fuel cell vehicles and portable power systems. Research has shown that 5-layer MEAs can provide improved performance under different operating conditions, making them a preferred choice in the industry.
It can be used to distinguish between proteins secreted by cells in culture and serum contaminants. [9] It has also been adapted as a 'forward+reverse' SILAC method for simultaneous labeling of host and microbe, which enables the study of host-microbe interactions. [10] Standardized protocols of SILAC for various applications have also been ...
A sperm cell can contain up to 50,000 toroid-shaped structures in its nucleus with each toroid containing about 50 kilobases. [4] Before the toroid is formed, histones are removed from the DNA by transition nuclear proteins, so that protamine can condense it. The effects of this change are 1) an increase in sperm hydrodynamics for better flow ...