Search results
Results from the WOW.Com Content Network
Typically, tRNAs genes from Bacteria are shorter (mean = 77.6 bp) than tRNAs from Archaea (mean = 83.1 bp) and eukaryotes (mean = 84.7 bp). [31] The mature tRNA follows an opposite pattern with tRNAs from Bacteria being usually longer (median = 77.6 nt) than tRNAs from Archaea (median = 76.8 nt), with eukaryotes exhibiting the shortest mature ...
Competitive RT-PCR technique is used for absolute quantification. It involves the use of a synthetic “competitor” RNA that can be distinguished from the target RNA by a small difference in size or sequence. It is important for the design of the synthetic RNA be identical in sequence but slightly shorter than the target RNA for accurate results.
Transcription-mediated amplification has several advantages compared to other amplification methods including: TMA is isothermal, meaning it is performed at constant temperature. As such, a water bath or heat block can be used instead of a thermal cycler. TMA produces RNA amplicon rather than DNA amplicon.
RNA-Seq refers to the combination of a high-throughput sequencing methodology with computational methods to capture and quantify transcripts present in an RNA extract. [10] The nucleotide sequences generated are typically around 100 bp in length, but can range from 30 bp to over 10,000 bp depending on the sequencing method used.
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA. It is used to diagnose infectious disease caused by RNA viruses. [1] It combines LAMP [2] DNA-detection with reverse transcription, making cDNA from RNA before running the reaction. [3]
Chaz Lanier scored 29 points to lead No. 1 Tennessee over No. 23 Arkansas 76-52 on Saturday and tie for the best start to a season in program history. The Volunteers (14-0, 1-0 Southeastern ...
Small RNA that is activated by SgrR in Escherichia coli during glucose-phosphate stress shRNA: short hairpin RNA - siRNA: small interfering RNA - SL RNA spliced leader RNA multiple families: SmY RNA: mRNA trans-splicing RF01844: Small nuclear RNAs found in some species of nematode worms, thought to be involved in mRNA trans-splicing snoRNA ...
Experts say vehicle-based attacks are simple for a 'lone wolf' terrorist to plan and execute, and challenging for authorities to prevent.