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Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope when stained. However, when unstained, the nucleus is visible and it usually has two lobes . [ 7 ] The mast cell , another granulocyte, is similar in appearance and function.
A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin.
Other widely used metachromatic stains include the family of Romanowsky stains that also contain thiazine dyes: the white cell nucleus stains purple, basophil granules intense magenta, whilst the cytoplasms (of mononuclear cells) stains blue, which is called the Romanowsky effect. The absence of color change in staining is named orthochromasia.
There are four types of granulocytes (full name polymorphonuclear granulocytes): [3] Basophils; Eosinophils; Neutrophils; Mast cells; Except for the mast cells, their names are derived from their staining characteristics; for example, the most abundant granulocyte is the neutrophil granulocyte, which has neutrally staining cytoplasmic granules.
Violet/purple granules Neutrophils: Blue nucleus, pink cytoplasm, violet granules Eosinophils: Blue nucleus, blue cytoplasm, red granules Basophils: Purple/dark blue nucleus, violet granules Monocyte: Violet nucleus, light blue cytoplasm Bacteria and fungi: Dark blue Cytoplasm, collagen and muscle: Various shades of pink, orange, yellow and ...
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. [1] These basophils are further classified by the hormones they produce.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Basophilia is the condition of having greater than 200 basophils/μL in the venous blood. [1] Basophils are the least numerous of the myelogenous cells, and it is rare for their numbers to be abnormally high without changes to other blood components.